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- 产品名称：重组大鼠脑多巴胺神经营养因子蛋白
- 产品型号：rRtCDNF
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-07-11
- 在线询价
简单介绍
重组大鼠脑多巴胺神经营养因子蛋白与其它公司提供的重组蛋白不同，rRtCDNF蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组大鼠脑多巴胺神经营养因子蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组大鼠脑多巴胺神经营养因子蛋白
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Species
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Rat
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Accession
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P0C5I0
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GeneID
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361276
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Source
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Escherichia coli.
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Molecular Weight
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重组大鼠脑多巴胺神经营养因子蛋白Approximately 18.8 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.
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Quantity
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5µg/25µg/1000µg
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AA Sequence
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QGLEAGVRSR ADCEVCKEFL NRFYNSLLTR GIDFSVDTIE EELISFCADT KGKENRLCYY LGATKDSATK ILGEVTRPMS VHMPTVKICE KLKKMDSQIC ELKYEKKLDL ESVDLWKMRV AELKQILHSW GEECRACAEK HDYVNLIKEL APKYVETRPQ TEL
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Purity
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重组大鼠脑多巴胺神经营养因子蛋白> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. It is able to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons when immobilized at 5 - 25 µg/mL on a nitrocellulose-coated microplate.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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重组大鼠脑多巴胺神经营养因子蛋白Less than 0.1 EU/µg of rRtCDNF as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组大鼠脑多巴胺神经营养因子蛋白
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SDS-PAGE
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