|
Synonyms
|
Za11
|
|
Species
|
Rat
|
|
Accession
|
A3QPB9
|
|
GeneID
|
365769
|
|
Source
|
Escherichia coli.
|
|
Molecular Weight
|
重组大鼠白细胞介素-21蛋白Approximately 15.2 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.
|
|
Quantity
|
2µg/10µg/1000µg
|
|
AA Sequence
|
HKSSPQRPDH LLIRLRHLMD IVEQLKIYEN DLDPELLTAP QDVKGQCEHE AFACFQKAKL KPSNTGNNKT FINDLLAQLR RRLPAKRTGN KQRHMAKCPS CDLYEKKTPK EFLERLKWLL QKMIHQHLS
|
|
Purity
|
> 96 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
重组大鼠白细胞介素-21蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
|
|
Endotoxin
|
Less than 1 EU/μg of rRtIL-21 as determined by LAL method.
|
|
Reconstitution
|
重组大鼠白细胞介素-21蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重组大鼠白细胞介素-21蛋白
|
|
|
SDS-PAGE
|
|
|
Reference
|
1. Pistoia VandCocco C. 2009. J Leukoc Biol, 85: 739-43.
2. Ertelt JM, Johanns TM, Rowe JH, et al. 2010. Immunology, 131: 183-91.
3. Denman CJ, Senyukov VV, Somanchi SS, et al. 2012. PLoS One, 7: e30264.
4. Spolski R, Wang L, Wan CK, et al. 2012. J Immunol, 188: 1924-32.
|
|
Background
|
Rat IL-21 is produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxin T cells and natural killer cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation with IL-4 of IgG production, and induction of apoptotic effects in naïve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, it promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies. Mature rat IL-21 shares 88% a.a. sequence identity with mouse IL-21.
|
