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- 产品名称：重组大鼠肝素结合性EGF样生长因子蛋白
- 产品型号：rRtHB-EGF
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-08-31
- 在线询价
简单介绍
重组大鼠肝素结合性EGF样生长因子蛋白与其它公司提供的重组蛋白不同，rRtHB-EGF蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组大鼠肝素结合性EGF样生长因子蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组大鼠肝素结合性EGF样生长因子蛋白
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Synonyms
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HBEGF, DT-R
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Species
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Rat
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Accession
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Q06175
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GeneID
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25433
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Source
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Escherichia coli.
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Molecular Weight
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重组大鼠肝素结合性EGF样生长因子蛋白Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
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Quantity
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10µg/50µg/1000µg
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AA Sequence
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DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组大鼠肝素结合性EGF样生长因子蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 300 mM NaCl, pH 7.4, 5 % trehalose.
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Endotoxin
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重组大鼠肝素结合性EGF样生长因子蛋白Less than 1 EU/μg of rRtHB-EGF as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组大鼠肝素结合性EGF样生长因子蛋白
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Reference
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1. Jin K, Mao XO, Sun Y, et al. 2002. J Neurosci. 22:5365-73.
2. Higashiyama S, Abraham JA, Miller J, et al. 1991. Science. 251:936-9.
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Background
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Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Rat HB-EGF shares about 76 % a.a. sequence identity with human HB-EGF.
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