- 如果您对该产品感兴趣的话，可以
- 产品名称：重组大鼠血小板衍生生长因子-AA蛋白
- 产品型号：rRtPDGF-AA
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-07-12
- 在线询价
简单介绍
重组大鼠血小板衍生生长因子-AA蛋白与其它公司提供的重组蛋白不同，rRtPDGF-AA蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组大鼠血小板衍生生长因子-AA蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组大鼠血小板衍生生长因子-AA蛋白
|
Species
|
Rat
|
|
Accession
|
P28576
|
|
GeneID
|
25266
|
|
Source
|
Escherichia coli.
|
|
Molecular Weight
|
Approximately 25.3 kDa, a disulfide-linked homodimeric protein containing two 111 amino acid residues polypeptide (A chain).
|
|
Quantity
|
2µg/10µg/1000µg
|
|
AA Sequence
|
重组大鼠血小板衍生生长因子-AA蛋白MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R
|
|
Purity
|
> 98 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.
|
|
Physical Appearance
|
重组大鼠血小板衍生生长因子-AA蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
|
|
Endotoxin
|
Less than 0.1 EU/μg of rRtPDGF-AA as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
重组大鼠血小板衍生生长因子-AA蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重组大鼠血小板衍生生长因子-AA蛋白
|
|
|
SDS-PAGE
|
|
