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Synonyms
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CXC Chemokine RTCK1, Chemokine (C-X-C Motif) Ligand 7, Isoform CRA_b, Protein Ppbp.
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Species
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Rat
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Source
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Escherichia coli.
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Molecular Weight
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Approximately 6.8 kDa, a single non-glycosylated polypeptide chain containing 62 amino acids.
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Quantity
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重组大鼠嗜中性粒细胞活化肽2 / CXCL7蛋白2µg/10µg/1000µg
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AA Sequence
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IELRCRCTNT LSGIPLNSIS RVNVFRPGAH CDNVEVIATL KNGKEVCLDP TAPMIKKIVK KI
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组大鼠嗜中性粒细胞活化肽2 / CXCL7蛋白Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 0.1-1.0 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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重组大鼠嗜中性粒细胞活化肽2 / CXCL7蛋白Less than 1 EU/µg of rRtNAP-2/CXCL7 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组大鼠嗜中性粒细胞活化肽2 / CXCL7蛋白
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SDS-PAGE
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Reference
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1. Hristov M, Zernecke A, Bidzhekov K, et al. 2007. Circ Res. 100:590-7.
2. Majumdar S, Gonder D, Koutsis B, et al. 1991. J Biol Chem. 266:5785-9.
3. Krijgsveld J, Zaat SA, Meeldijk J, et al. 2000. J Biol Chem. 275:20374-81.
4. Piccardoni P, Evangelista V, Piccoli A, et al. 1996. Thromb Haemost. 76:780-5.
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Background
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CXCL7 is a small cytokine which is also an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein belonging to the CXC chemokine family and it is released in large amounts from platelets following their activation. CXCL7 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. Recombinant Rat CXCL7 contains 62 amino acids which is a single non-glycosylated polypeptide chain. In addition, The rat CXCL7 shares 58 % and 77 % a.a. sequence identity with human and mouse CXCL7.
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