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Species
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Rat
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Accession
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Q9QZD1
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GeneID
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24772
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Source
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Escherichia coli.
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Molecular Weight
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重组大鼠基质细胞衍生因子-1 alpha / CXCL12 alpha蛋白Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组大鼠基质细胞衍生因子-1 alpha / CXCL12 alpha蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rRtSDF-1α/CXCL12α as determined by LAL method.
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Reconstitution
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重组大鼠基质细胞衍生因子-1 alpha / CXCL12 alpha蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组大鼠基质细胞衍生因子-1 alpha / CXCL12 alpha蛋白
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Reference
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1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500.
2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9.
3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51.
4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9.
5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23.
6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703.
7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.
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Background
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CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. Rat CXCL12 is expressed as two isoforms that differ only in the C-terminal tail. And both SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, rat CXCL12α shares approximately 96% amino acid sequence identity with human CXCL12α.
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