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Synonyms
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Dendritic Cell and Monocyte Chemokine-like Protein, DMC
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Species
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Rat
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Source
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Escherichia coli.
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Molecular Weight
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重组蛋白价格Approximately 11.5 kDa, a single non-glycosylated polypeptide chain containing 97 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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SPNQEVARHH GDQHQAPRRW LWEGGQECDC KDWSLRVSKR KTTAVLEPPR KQCPCDHVKG SEKKNRRQKH HRKSQRPSRT CQQFLKRCQL ASFTLPL
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组蛋白价格Fully biologically active when compared to standard. The ED50 as determined by its ability to induce VEGF expression using murine endothelial cells is less than 5.0 μg/ml, corresponding to a specific activity of > 200 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4.
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Endotoxin
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重组蛋白价格Less than 1 EU/µg of rRtVCC-1/CXCL17 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组蛋白价格
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Reference
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1. WY Lee, CJ Wang, TY Lin, et al. 2013. Am J Physiol Endocrinol Metab, 304: E32-40.
2. A Matsui, H Yokoo, Y Negishi, et al. 2012. PLoS One, 7: e44080.
3. AM Burkhardt, KP Tai, JP Flores-Guiterrez, et al. 2012. J Immunol, 188: 6399-406.
4. N Hiraoka, R Yamazaki-Itoh, Y Ino, et al. 2011. Gastroenterology, 140: 310-21.
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Background
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重组蛋白价格also named Dendritic Cell and Monocyte Chemokine-like Protein (DMC) and VEGF Co-regulated Chemokine 1 (VCC-1), is a small cytokine belonging to the CXC chemokine family. It is constitutively expressed in the lung and can be detected in trachea, stomach and skeletal muscle. CXCL17 attracts dendritic cells and monocytes and is regulated in tumors. It may be a housekeeping chemokine regulating recruitment of nonactivated blood monocytes and immature dendritic cells into tissues and may play a role in the innate defense against infections. Mature human CXCL17 shares 73 %, 71 % and 64 % amino acid sequence identity with bovine, mouse and rat DMC, respectively.
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