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Synonyms
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CK-beta-6, MPIF-2, Small-inducible Cytokine A24
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Species
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Rat
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Source
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Escherichia coli.
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Molecular Weight
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Approximately 10.5 kDa, a single non-glycosylated polypeptide chain consisting of an N-terminal Methionine and the mature rat Eotaxin-2.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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重组大鼠嗜酸性粒细胞趋化因子-2 / CCL24蛋白MPTGSVTIPS SCCVTFISKK IPVNRVISYQ LANGSICPKA GVIFITKKGH KICTDPKLPW VQKHIKNLDA KRNQPSEGAK ALGPKFVIQK LRGNSTKV
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Purity
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> 96 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-250 ng/ml.
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Physical Appearance
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重组大鼠嗜酸性粒细胞趋化因子-2 / CCL24蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/µg of rRtEotaxin-2/CCL24 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组大鼠嗜酸性粒细胞趋化因子-2 / CCL24蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组大鼠嗜酸性粒细胞趋化因子-2 / CCL24蛋白
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SDS-PAGE
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Reference
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1. Owczarek W, Paplinska M, Targowski T, et al. 2010. Cytokine, 50: 181-5.
2. Ben-Yehuda C, Bader R, Puxeddu I, et al. 2008. Exp Lung Res, 34: 467-79.
3. Schaefer D, Meyer JE, Pods R, et al. 2006. Int Arch Allergy Immunol, 140: 205-14.
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Background
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CCL24, also named MPIF-2, Eotaxin-2 and Ckβ6, is a novel CC chemokine recently identified. It is a secreted protein, encoded by CCL24 gene, and produced by activated monocytes and T lymphocytes. CCL24 signals through the CCR3 receptor and has functions of chemotactic activity for resting T-lymphocytes and eosinophils, but none for monocytes and activated lymphocytes. The plasma levels of CCL24 and the aspirin-exacerbated respiratory disease (such as asthma) morbidity rate have positive correlation.
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