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Synonyms
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GRO-alpha, Platelet-derived Growth Factor-inducible Protein KC
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Species
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Rat
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Accession
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P14095
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GeneID
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81503
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Source
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重组大鼠细胞因子诱导中性粒细胞趋化物1 / CXCL1蛋白Escherichia coli.
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Molecular Weight
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Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 72 amino acids.
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Quantity
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5µg/25µg/1000µg
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AA Sequence
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重组大鼠细胞因子诱导中性粒细胞趋化物1 / CXCL1蛋白APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml.
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Physical Appearance
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重组大鼠细胞因子诱导中性粒细胞趋化物1 / CXCL1蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rRtCINC-1/CXCL1 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组大鼠细胞因子诱导中性粒细胞趋化物1 / CXCL1蛋白
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Reference
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1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6.
2. Richmond A, Thomas HG. 1988. J Cell Biochem. 36:185-98.
3. Iida N, Grotendorst GR. 1990. Mol Cell Biol. 10:5596-9.
4. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.
5. Moser B, Clark-Lewis I, Zwahlen R, et al. 1990. J Exp Med. 171:1797-802.
6. Devalaraja RM, Nanney LB, Du J, et al. 2000. J Invest Dermatol. 115:234-44.
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Background
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Rat CXCL1, also known as CINC-1, is belonging to the CXC chemokine family. It is encoded by the GRO gene now designated CXCL1. It was originally purified from media conditioned by IL-1β stimulated rat kidney epithelioid cells. CINC-1 is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. CXCL1 is secreted by human melanoma cells, and also expressed by macrophages, neutrophils and epithelial cells. The functional receptor for CXCL1 has been identified as CXCR2. CXCL1 has chemotactic activity for neutrophils, and plays a role in inflammation and wound healing. Amino acid sequence of rat CXCL1 is approximately 68 % identical to the human CXCL1.
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