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Species
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Rat
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Accession
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Q9ERE0
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GeneID
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117518
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Source
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Escherichia coli.
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Molecular Weight
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重组大鼠胸腺和激活调节的趋化因子/ CCL17蛋白Approximately 8.1 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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ARATNVGREC CLDYFKGAIP IRKLVTWFRT SVECPKDAIV FETVQGRLIC TDPKDKHVKK AIRHLKNQRL
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组大鼠胸腺和激活调节的趋化因子/ CCL17蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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重组大鼠胸腺和激活调节的趋化因子/ CCL17蛋白Less than 0.1 EU/μg of r rRtTARC/CCL17 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组大鼠胸腺和激活调节的趋化因子/ CCL17蛋白
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SDS-PAGE
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Reference
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1. Loftus BJ, Kim UJ, Sneddon VP, et al. 1999. Genomics, 60: 295-308.
2. Imai T, Yoshida T, Baba M, et al. 1996. J Biol Chem, 271: 21514-21.
3. Jakubzick C, Wen H, Matsukawa A, et al. 2004. Am J Pathol, 165: 1211-21.
4. Shimada Y, Takehara K, Sato S. 2004. J Dermatol Sci, 34: 201-8.
5. Sumiyoshi K, Nakao A, Setoguchi Y, et al. 2003. J Dermatol Sci, 31: 53-8.
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Background
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CCL17 also known as thymus and activation-related chemokine (TARC) is encoded by the CCL17 gene located on the chromosome 16. It is expressed by thymus cells constitutively and phytohemagglutinin-stimulated peripheral blood mononuclear cells transiently. CCL17 signals through the chemokine receptors CCR4 and CCR8 and displays chemotactic activity for T lymphocytes and some other leukocytes. It plays an important role in skin diseases such as atopic dermatitis, bullous pemphigoid and mycosis fungoides. CCL17 has approximately 24 – 29 % amino acid sequence identity with RANTES, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3 and I-309.
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