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Synonyms
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MIF, Glutathione-binding 13 kDa Protein, L-dopachrome Isomerase, L-dopachrome Tautomerase, Phenylpyruvate Tautomerase
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Species
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Rat
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Accession
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P30904
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GeneID
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81683
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Source
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Escherichia coli.
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Molecular Weight
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重组大鼠迁移抑制因子蛋白Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 115 amino acids.
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Quantity
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10µg/50µg/1000µg
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AA Sequence
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MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Test in process.
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Physical Appearance
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重组大鼠迁移抑制因子蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, pH 8.0, 150 mM NaCl, 3 % trehalose.
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Endotoxin
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Less than 1 EU/µg of rRtMIF as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组大鼠迁移抑制因子蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组大鼠迁移抑制因子蛋白
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SDS-PAGE
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Reference
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1. WY Weiser, PA Temple, JS Witek-Giannotti, et al. 1989. Proc Natl Acad Sci U S A, 86: 7522-6.
2. CA Kozak, MC Adamson, CE Buckler, et al. 1995. Genomics, 27: 405-11.
3. DF LarsonandK Horak. 2006. Crit Care, 10: 138.
4. HW Sun, J Bernhagen, R Bucala, et al. 1996. Proc Natl Acad Sci U S A, 93: 5191-6.
5. M Oddo, T Calandra, R Bucala, et al. 2005. Infect Immun, 73: 3783-6.
6. M Emonts, FC Sweep, N Grebenchtchikov, et al. 2007. Clin Infect Dis, 44: 1321-8.
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Background
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Macrophage migration inhibitory factor (MIF or MMIF), also named as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase, is a protein encoded by the MIF gene. It is released from white blood cells by bacterial antigen stimulation to trigger an acute immune response, or by glucocorticoids to counter-act the inhibitory effects of glucocorticoids on immune system. MIF is a homotrimer of which each subunit contains 115 amino acids. As mentioned above, MIF is involved in the innate immune response to bacterial pathogens and counter-acts the anti-inflammatory activity of glucocorticoids. Furthermore, it also plays a role as mediator in regulating the function of macrophages in host defense and has phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro. Rat MIF is 99 %, 90 %, 89 %, and 89 % a.a. identical to human, murine, porcine and bovine, respectively.
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