重组猪白细胞介素-1β蛋白 rPoIL-1β 上海康朗生物科技有限公司

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产品名称：重组猪白细胞介素-1β蛋白
产品型号：rPoIL-1β
产品展商：KALANG
产品文档：无相关文档
发布时间：2018-09-06
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简单介绍

重组猪白细胞介素-1β蛋白与其它公司提供的重组蛋白不同，rPoIL-1β蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组猪白细胞介素-1β蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.

产品描述

重组猪白细胞介素-1β蛋白

Synonyms	IL-1 beta
Species	Porcine
Accession	P26889
GeneID	397122
Source	Escherichia coli.
Molecular Weight	重组猪白细胞介素-1β蛋白Approximately17.6 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Quantity	2µg/10µg/1000µg
AA Sequence	ANVQSMECKL QDKDHKSLVL AGPHMLKALH LLTGDLKREV VFCMSFVQGD DSNNKIPVTL GIKGKNLYLS CVMKDNTPTL QLEDIDPKRY PKRDMEKRFV FYKTEIKNRV EFESALYPNW YISTSQAEQK PVFLGNSKGR QDITDFTMEV LSP
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重组猪白细胞介素-1β蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine D10S cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 10⁵ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 3 % trehalose.
Endotoxin	Less than 1 EU/μg of rPoIL-1β as determined by LAL method.
Reconstitution	重组猪白细胞介素-1β蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组猪白细胞介素-1β蛋白
SDS-PAGE
Reference	1. Bankers-Fulbright, J.L., K.R. Kalli, and D.J. McKean. 1996. Life Sci, 59: 61-83. 2. Dinarello, C.A. 1997. Semin Oncol, 24: S9-81-S9-93. 3. Martinon, F., and J. Tschopp. 2007. Cell Death Differ, 14: 10-22. 4. Auron, P.E., A.C. Webb, L.J. Rosenwasser, et al. 1984. Proc Natl Acad Sci U S A, 81: 7907-11.
Background	Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. Recombinant porcine interleukin-1 beta is a 17 kDa protein containing 153 amino acid residues and it shares 63 % - 70 % a.a. sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL1β.