巨噬细胞集落刺激因子蛋白 rCaGM-CSF 上海康朗生物科技有限公司

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产品名称：重组犬粒细胞 - 巨噬细胞集落刺激因子蛋白
产品型号：rCaGM-CSF
产品展商：KALANG
产品文档：无相关文档
发布时间：2018-07-20
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简单介绍

重组犬粒细胞 - 巨噬细胞集落刺激因子蛋白与其它公司提供的重组蛋白不同，rCaGM-CSF蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组犬粒细胞 - 巨噬细胞集落刺激因子蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.

产品描述

重组犬粒细胞 - 巨噬细胞集落刺激因子蛋白

Synonyms	Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α
Species	Canine
Accession	P48749
GeneID	403923
Source	Escherichia coli.
Molecular Weight	重组犬粒细胞 - 巨噬细胞集落刺激因子蛋白Approximately 14.2 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.
Quantity	5µg/20µg/1000µg
AA Sequence	APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPE GPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQN INFKSFKENL KDFLFNIPFD CWKPVKK
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重组犬粒细胞 - 巨噬细胞集落刺激因子蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁵ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rCaGM-CSF as determined by LAL method.
Reconstitution	重组犬粒细胞 - 巨噬细胞集落刺激因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组犬粒细胞 - 巨噬细胞集落刺激因子蛋白
Reference	1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3. 2. 1989. N Engl J Med, 320: 253-4. 3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101. 4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27. 5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.
Background	Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation. It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages. GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate. Additionally, it can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. It is reported that GM-CSF has no biological effects across species.