说明:
CD45RO 微珠用于直接磁性标记**细胞亚群。CD45RO表达于CD4+和CD8+ 记忆性T细胞、CD4+效应性T细胞。此外还见于单核细胞、巨噬细胞、髓样DC、粒细胞。CD45RO抗体识别白细胞共同抗原 (LCA) 的180 kDa异构体。
应用:
CD45RO 微珠用于阳性分选或去除CD45RO+ 细胞,还可以与MACS CD4+、CD8+T细胞分选试剂盒或多选试剂盒联合应用,分选CD45RO+ T细胞亚群。CD45RO微珠也可用于从分选出的CD4+ 或CD8+T细胞中,通过去除CD45RO+ 细胞,得到CD45RA+ 细胞。CD45RO微珠分选得到的细胞可用于多种研究,如:细胞因子表达和受体信号研究。
分选柱:阳性选择可用MS、LS、XS或autoMACSTM分选柱;去除分选可用LD、CS、D或autoMACS分选柱。
CD4+ cells were enriched from PBMCs by using the CD4 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RO MicroBeads. CD4+CD45RO+ effector and memory T helper cells were isolated by positive selection. Pre-enriched CD4+ cells CD4+CD45RO+cells
CD4+ cells were enriched from PBMCs by using the CD4 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RO MicroBeads. CD4+CD45RO+ effector and memory T helper cells were isolated by positive selection.
Pre-enriched CD4+ cells CD4+CD45RO+cells