说明:
CD45RA 微珠用于直接磁性标记**细胞亚群。CD45RA表达于初始性CD4+ T细胞、初始性CD8+ T细胞、CD8+效应性T细胞。此外还见于B细胞亚群、NK细胞亚群、浆样DC。CD45RA抗体识别白细胞共同抗原 (LCA) 的220 kDa异构体。
应用:
CD45RA 微珠用于阳性分选或去除CD45RA+ 细胞,还可以与MACS CD4+ 、CD8+、全T细胞分选试剂盒6,7或CD4+和CD8+多选试剂盒联合应用,分选CD45RA+ T细胞亚群。CD45RA微珠也可用于从分选出的CD4+ 或CD8+T细胞中,通过去除CD45RA+ 细胞,得到CD45RO+ 细胞。CD45RA微珠分选得到的细胞可用于多种研究,如:细胞因子表达和受体信号研究。
分选柱:阳性选择可用MS、LS、XS或autoMACSTM分选柱;去除分选可用LD、CS、D或autoMACS分选柱。
CD8+ cells were enriched from PBMCs by using the CD8 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RA MicroBeads. Naive CD8+CD45RA+ T cells were isolated by positive selection. Pre-enriched CD8+ cells CD8+CD45RA+ cells
CD8+ cells were enriched from PBMCs by using the CD8 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RA MicroBeads. Naive CD8+CD45RA+ T cells were isolated by positive selection.
Pre-enriched CD8+ cells CD8+CD45RA+ cells