颗粒酶H(GZMH)重组蛋白
适用生物 Homo sapiens (Human，人)
颗粒酶H(GZMH)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 29.2kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Granzyme H (GZMH)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
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Organism species
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Homo sapiens (Human)
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Product No.
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HZL575Hu01
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Source
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Prokaryotic expression
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Host
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E.coli
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Purity
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> 95%
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UOM
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50ug
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Predicted Molecular Mass
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29.2kDa
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Predicted isoelectric point
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9.9
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Applications
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SDS-PAGE; WB; ELISA; IP.
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Endotoxin Level
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<1.0EU per 1µg (determined by the LAL method)
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Subcellular Location
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Cytoplasmic granule.
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Residues
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Glu19~Leu246 (Accession # P20718) with two N-terminal Tags, His-tag and T7-tag
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Formulation
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Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
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SEQUENCES
颗粒酶H(GZMH)重组蛋白 The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EE IIGGHEAKPH SRPYMAFVQF LQEKSRKRCG GILVRKDFVL TAAHCQGSSI NVTLGAHNIK EQERTQQFIP VKRPIPHPAY NPKNFSNDIM LLQLERKAKW TTAVRPLRLP SSKAQVKPGQ LCSVAGWGYV SMSTLATTLQ EVLLTVQKDC QCERLFHGNY SRATEICVGD PKKTQTGFKG DSGGPLVCKD VAQGILSYGN KKGTPPGVYI KVSHFLPWIK RTMKRL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
颗粒酶H(GZMH)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
颗粒酶H(GZMH)重组蛋白 Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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