钙非依赖性磷脂酶A2(iPLA2)重组蛋白
适用生物 Homo sapiens (Human，人)
钙非依赖性磷脂酶A2(iPLA2)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 28.5kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Phospholipase A2, Calcium Independent (iPLA2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
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Organism species
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Homo sapiens (Human)
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Product No.
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HZA411Hu01
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Source
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Prokaryotic expression
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Host
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E.coli
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Purity
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> 95%
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UOM
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50ug
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Predicted Molecular Mass
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28.5kDa
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Predicted isoelectric point
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9.4
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Applications
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SDS-PAGE; WB; ELISA; IP.
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Endotoxin Level
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<1.0EU per 1µg (determined by the LAL method)
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Subcellular Location
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Cytoplasm
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Residues
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Arg484~Ile701 (Accession # O60733) with two N-terminal Tags, His-tag and T7-tag
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Formulation
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Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
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SEQUENCES
钙非依赖性磷脂酶A2(iPLA2)重组蛋白The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RGMYFRM KDEVFRGSRP YESGPLEEFL KREFGEHTKM TDVRKPKVML TGTLSDRQPA ELHLFRNYDA PETVREPRFN QNVNLRPPAQ PSDQLVWRAA RSSGAAPTYF RPNGRFLDGG LLANNPTLDA MTEIHEYNQD LIRKGQANKV KKLSIVVSLG TGRSPQVPVT CVDVFRPSNP WELAKTVFGA KELGKMVVDC CTDPDGRAVD RARAWCEMVG I
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
钙非依赖性磷脂酶A2(iPLA2)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
钙非依赖性磷脂酶A2(iPLA2)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
