产品描述
STAT3报告基因是自主研发的用于检测STAT3二聚体和STAT1/STAT3异质二聚体的转录活性水平为目的的报告基因。激活STAT3后形成STAT3二聚体和STAT1/STAT3异质二聚体，它们与SIS-inducible element（SIE）反应元件进行相互作用调控细胞的生长和凋亡。STAT3(signal transducers and activators of transduction-3)报告基因与多种恶性肿瘤的发生、发展和侵袭转移关系密切。
STAT3报告基因主要用于监控细胞STAT3信号通路中的STAT3转录活性、肿瘤****的研发以及基因过表达和RNAi的表型分析等。
pGMSTAT3-GFP是改造后的哺乳动物真核表达载体，在其多克隆位点插入了多个STAT3结合位点，可以高效地检测STAT3的激活水平。由于载体采用了GFP作为报告基因，更便于后续的检测。同时，对载体中预测出的其它转录因子以外的结合位点进行了适当的突变，增加了质粒的转录因子结合特异性。另外，由于质粒体积减小，使得STAT3-GFP报告基因更易于转染。STAT3 GFP Reporter Plasmid（STAT3-GFP报告基因质粒）
质粒图谱
STAT3 GFP Reporter Plasmid（STAT3-GFP报告基因质粒）pGMSTAT3 -GFP质粒测序引物
5’-TAGCAAAATAGGCTGTCCC-3’
使用说明
pGMSTAT3-GFP可以采用常规转染方法转染哺乳动物细胞。
注意事项
1）本质粒未经允许不得用于任何商业用途，也不得移交给订货人实验室以外的任何人或单位。
2）为了您的健康，实验操作时请穿实验服和带一次性手套。STAT3 GFP Reporter Plasmid（STAT3-GFP报告基因质粒）
保存条件
冰袋运输。-20℃保存。
参考文献
[1] Kang Y, et al. The radio-sensitizing effect of xanthohumol is mediated by STAT3 and EGFR suppression in doxorubicin-resistant MCF-7 human breast cancer cells. Biochim Biophys Acta. 2012 Dec 16. pii: S0304-4165(12)00351-0.
[2] Teng J, et al. Bx-dependent activation of Twist mediates STAT3 Control of epithelium-mesenchymal transition of liver cells.J Cell Biochem. 2012 Nov 13. doi: 10.1002/jcb.24450.
[3]Villa S, et al. The influence of the substitution pattern on the molecular conformation of ureido-1,2,5-oxadiazoles, related to STAT3 inhibitors: chemical behavior and structural investigation.Chem Biodivers. 9(7):1240-53(2012).
[4] Han L, et al. MicroRNA-21 expression is regulated by β-catenin/STAT3 pathway and promotes glioma cell invasion by direct targeting RECK.CNS Neurosci Ther.18(7):573-83(2012).
[5] Kwon MJ, Ma J, Ding Y, Wang R, Sun Z.Protein kinase C-θ promotes Th17 differentiation via upregulation of Stat3.J Immunol.;188(12):5887-97(2012).
[6]Seo HS, et al. Apigenin induces apoptosis via extrinsic pathway, inducing p53 and inhibiting STAT3 and NFκB signaling in HER2-overexpressing breast cancer cells.Mol Cell Biochem.366(1-2):319-34(2012).
[7] Lim EJ, et al. Methylsulfonylmethane suppresses breast cancer growth by down-regulating STAT3 and STAT5b pathways.PLoS One. 7(4):e33361(2012).
[8] Hahm ER, et al. Sulforaphane inhibits constitutive and interleukin-6-induced activation of signal transducer and activator of transcription 3 in prostate cancer cells.Cancer Prev Res (Phila). 3(4):484-94(2010)
[9] Lin L, et al. A novel small molecule, LLL12, inhibits STAT3 phosphorylation and activities and exhibits potent growth-suppressive activity in human cancer cells.Neoplasia.12(1):39-50(2010).
[10] Shin DS, et al. Cryptotanshinone inhibits constitutive signal transducer and activator of transcription 3 function through blocking the dimerization in DU145 prostate cancer cells.Cancer Res. 69(1):193-202(2009).
[11] Duan Z, et al. Signal transducers and activators of transcription 3 pathway activation in drug-resistant ovarian cancer.Clin Cancer Res. 12(17):5055-63(2006).STAT3 GFP Reporter Plasmid（STAT3-GFP报告基因质粒）
