Diaphanous同源物1(DIAPH1)重组蛋白
适用生物 Homo sapiens (Human，人)
Diaphanous同源物1(DIAPH1)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 54.5kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Diaphanous Homolog 1 (DIAPH1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
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Organism species
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Homo sapiens (Human)
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Product No.
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HZJ265Hu01
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Source
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Prokaryotic expression
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Host
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E.coli
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Purity
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> 95%
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UOM
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50ug
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Predicted Molecular Mass
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54.5kDa
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Predicted isoelectric point
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5.7
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Applications
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SDS-PAGE; WB; ELISA; IP.
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Endotoxin Level
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<1.0EU per 1µg (determined by the LAL method)
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Subcellular Location
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Cell membrane. Cell projection, ruffle membrane. Cytoplasm, cytoskeleton.
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Residues
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Asp389~Pro583 (Accession # O60610) with two N-terminal Tags, His-tag and GST-tag
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Formulation
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Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
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SEQUENCES
Diaphanous同源物1(DIAPH1)重组蛋白The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-DF NEVFQILLNT VKDSKAEPHF LSILQHLLLV RNDYEARPQY YKLIEECISQ IVLHKNGADP DFKCRHLQIE IEGLIDQMID KTKVEKSEAK AAELEKKLDS ELTARHELQV EMKKMESDFE QKLQDLQGEK DALHSEKQQI ATEKQDLEAE VSQLTGEVAK LTKELEDAKK EMASLSAAAI TVPPSVPSRA PVP
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Diaphanous同源物1(DIAPH1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Diaphanous同源物1(DIAPH1)重组蛋白Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
