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- 产品名称：甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体
- 产品型号：GAPDH
- 产品展商：HZbscience
- 产品文档：无相关文档
- 发布时间：2018-09-04
- 在线询价
简单介绍
甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体是针对GAPDH的兔多克隆抗体。在**组织化学染色和**印迹实验中能识别GAPDH。甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体丰富产品组合，其中包括超过 42,000 多种高品质抗体，并配套提供种类齐全的抗体相关产品。 我们的抗体测定结果经全球上千次的引用验证，有助于您在一系列抗体应用中获得优异的实验结果。
产品描述
甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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标记物无标记物
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**原甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体-
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缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
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性状液体
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浓度200ug/ml
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且适物种-
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应用甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体WB, ICC, IHC-P, IHC-F, ELISA
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Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: GAPDH, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant GAPDH (Gly2~Ser148) expressed in E.coli. Accession No.: RPB932Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MGHHHHHHSGSEF - GKVKVGVNG FGRIGR LVTR AAFNSGKV DI VAI NDPFI DL
NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG
VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIIS
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against GAPDH. It has been
selected for its ability to recognize GAPDH in immunohistochemical staining and
western blotting. 甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant GAPDH (Gly2~Ser148)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. 甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin- embedded liver tissue
Used in Western Blot, Sample:
Recombinant GAPDH, Human
