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- 产品名称：甲胎蛋白(αFP)多克隆抗体
- 产品型号：αFP
- 产品展商：HZbscience
- 产品文档：无相关文档
- 发布时间：2016-07-13
- 在线询价
简单介绍
甲胎蛋白(αFP)多克隆抗体是针对aFP的兔多克隆抗体。在**组织化学染色和**印迹实验中能识别aFP。甲胎蛋白(αFP)多克隆抗体丰富产品组合，其中包括超过 42,000 多种高品质抗体，并配套提供种类齐全的抗体相关产品。 我们的抗体测定结果经全球上千次的引用验证，有助于您在一系列抗体应用中获得优异的实验结果。
产品描述
甲胎蛋白(αFP)多克隆抗体
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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标记物甲胎蛋白(αFP)多克隆抗体无标记物
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**原RPA153Hu01-甲胎蛋白(aFP)重组蛋白
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缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
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性状液体
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浓度200ug/ml
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且适物种-
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应用甲胎蛋白(αFP)多克隆抗体WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
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Polyclonal Antibody to Alpha-Fetoprotein (aFP)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200µg/mL
UOM: 100µg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
Immunogen: RPA153Hu01-Recombinant Alpha-Fetoprotein (aFP)
[ APPLICATIONS ]
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. 甲胎蛋白(αFP)多克隆抗体[ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant aFP disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ IDENTIFICATION ]
A B
Figure 1. Western Blot
A. Sample: Recombinant aFP, Human B. Lane1: Human Liver Tissue Lane2: Human Lung Tissue Primary Ab: 3µg/mL Rabbit Anti-Human aFP Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SAA544Rb59)
A Figure 2. DAB staining on IHC-P Samples： A. Human Liver Tissue B. Human Liver Cancer Tissue C. Human Stomach Tissue C B甲胎蛋白(αFP)多克隆抗体
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