Bcl2关联死亡启动子(BAD)多克隆抗体

Bcl2关联死亡启动子(BAD)多克隆抗体 

• 来源多抗制备
• 宿主兔
• 效价-
• Ig类型IgG
• 纯化方式抗原特异性亲和纯化
• Bcl2关联死亡启动子(BAD)多克隆抗体标记物无标记物
• **原-
• 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
• 性状液体
• 浓度200ug/ml
• 且适物种-
• 应用WB, ICC, IHC-P, IHC-F, ELISA
• Bcl2关联死亡启动子(BAD)多克隆抗体Polyclonal Antibody to Bcl2 Associated Death Promoter (BAD)
  Organism Species: Homo sapiens (Human)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  9th Edition (Revised in Jul, 2013)
  [ PRODUCT INFORMATION ]
  Immunogen: BAD, Human
  Clonality: Polyclonal
  Host: Rabbit
  Immunoglobulin Type: IgG
  Purification: Affinity Chromatography.
  Applications: WB, ICC, IHC-P, IHC-F, ELISA
  Concentration: 200µg/mL
  UOM: 100µg
  Bcl2关联死亡启动子(BAD)多克隆抗体[ IMMUNOGEN INFORMATION ]
  Immunogen: Recombinant BAD (Met1~Gln168) expressed in E.coli. Accession No.: RPC337Hu01
  Sequence: The target protein is fused with two N-terminal Tags, His-tag and
  T7-tag and its sequence is listed below.
  MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MFQIPEFEPS EQEDSSSAER
  GLGPSPAG DG PSGSGK HHR Q APG L LWDAS H QQEQPTSSS H HGGAGAVEI R
  SR HSSYPAGT EDDEGMGEEP SPF RG RS RSA PP N LWAAQRY GRE LRRMS DE
  FV D S F K K G L P RP K S A G TAT Q M R Q S S SW T RV FQ SWW D R N L G RG S S A P S Q
  [ ANTIBODY SPECIFITY ]
  The antibody is a rabbit polyclonal antibody raised against BAD. It has been
  selected for its ability to recognize BAD in immunohistochemical staining and
  western blotting.
  [ APPLICATIONS ]
  Western blotting: 1:50-400
  Immunocytochemistry in formalin fixed cells: 1:50-500
  Immunohistochemistry in formalin fixed frozen section: 1:50-500
  Immunohistochemistry in paraffin section: 1:10-100
  Enzyme-linked Immunosorbent Assay: 1:100-200
  Optimal working dilutions must be determined by end user.
  [ IMAGES ]
  Bcl2关联死亡启动子(BAD)多克隆抗体[ CONTENTS ]
  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  [ QUALITY CONTROL ]
  Content: The quality control contains recombinant BAD (Met1~Gln168) disposed
  in loading buffer.
  Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
  5uL pe r well when used in enhan ced chemilumes cent (ECL ).
  Note: The quality control is specifically manufactured as the positive control.
  Not used for other purposes.
  Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
  Store at 4
  oC for frequent use. Stored at -20oC to -80
  oC in a manual defrost freezer
  for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  Used in DAB staining on fromalin fixed
  paraffin-embedded kidney tissue
  Used in Western Blot, Sample:
  Recombinant BAD, Human