90kDa热休克蛋白αB1(HSP90aB1)多克隆抗体

90kDa热休克蛋白αB1(HSP90aB1)多克隆抗体 

• 来源多抗制备
• 宿主兔
• 效价-
• Ig类型IgG
• 纯化方式抗原特异性亲和纯化
• 90kDa热休克蛋白αB1(HSP90aB1)多克隆抗体标记物无标记物
• **原-
• 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
• 性状液体
• 浓度200ug/ml
• 且适物种-
• 应用WB, ICC, IHC-P, IHC-F, ELISA
• 90kDa热休克蛋白αB1(HSP90aB1)多克隆抗体Polyclonal Antibody to Heat Shock Protein 90kDa Alpha B1 (HSP90aB1)
  Organism Species: Homo sapiens (Human)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  9th Edition (Revised in Jul, 2013)
  [ PRODUCT INFORMATION ]
  Immunogen: HSP90aB1, Human
  Clonality: Polyclonal
  Host: Rabbit
  Immunoglobulin Type: IgG
  Purification: Affinity Chromatography.
  Applications: WB, ICC, IHC-P, IHC-F, ELISA
  Concentration: 200µg/mL
  UOM: 100µg
  90kDa热休克蛋白αB1(HSP90aB1)多克隆抗体[ IMMUNOGEN INFORMATION ]
  Immunogen: Recombinant HSP90aB1 (Met1~Asp724) expressed in E.coli. Accession No.: RPD522Hu01
  Sequence: The target protein is fused with N-terminal His-Tag and its sequence
  is listed below.
  MGHHHHHHSGSEFELRRQ-MPEEVHHGEE EVETFAFQAE IAQLMSLIIN TFYSNKEIFL
  RELISNASDA LDKIRYESLT DPSKLDSGKE LKIDIIPNPQ ERTLTLVDTG IGMTKADLIN
  NLGTIAKSGT KAFMEALQAG ADISMIGQFG VGFYSAYLVA EKVVVITKHN DDEQYAWESS
  AGGSFTVRAD HGEPIGRGTK VILHLKEDQT EYLEERRVKE VVKKHSQFIG YPITLYLEKE
  REKEISDDEA EEEKGEKEEE DKDDEEKPKI EDVGSDEEDD SGKDKKKKTK KIKEKYIDQE
  ELNKTKPIWT RNPDDITQEEYGEFYKSLTN DWEDHLAVKH FSVEGQLEFR ALLFIPRRAP
  FDLFENKKKK NNIKLYVRRV FIMDSCDELI PEYLNFIRGV VDSEDLPLNI SREMLQQSKI
  LKVIRKNIVK KCLELFSELA EDKENYKKFY EAFSKNLKLG IHEDSTNRRR LSELLRYHTS
  QSGDEMTSLS EYVSRMKETQ KSIYYITGES KEQVANSAFV ERVRKRGFEV VYMTEPIDEY
  CVQQLKEFDG KSLVSVTKEG LELPEDEEEK KKMEESKAKF ENLCKLMKEI LDKKVEKVTI
  SNRLVSSPCC IVTSTYGWTA NMERIMKAQA LRDNSTMGYM MAKKHLEINP DHPIVETLRQ
  KAEADKNDKA VKDLVVLLFE TALLSSGFSL EDPQTHSNRI YRMIKLGLGI DEDEVAAEEP
  NAAVPDEIPP LEGDEDASRM EEVD
  [ ANTIBODY SPECIFITY ]
  The antibody is a rabbit polyclonal antibody raised against HSP90aB1. It has been
  selected for its ability to recognize HSP90aB1 in immunohistochemical staining
  and western blotting.
  90kDa热休克蛋白αB1(HSP90aB1)多克隆抗体[ APPLICATIONS ]
  Western blotting: 1:100-400
  Immunocytochemistry in formalin fixed cells: 1:100-500
  Immunohistochemistry in formalin fixed frozen section: 1:100-500
  Immunohistochemistry in paraffin section: 1:50-200
  Enzyme-linked Immunosorbent Assay: 1:100-200
  Optimal working dilutions must be determined by end user.
  [ IMAGES ]
  [ CONTENTS ]
  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  [ QUALITY CONTROL ]
  Content: The quality control contains recombinant HSP90aB1 (Met1~Asp724)
  disposed in loading buffer.
  Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
  5uL pe r well when used in enhan ced chemilumes cent (ECL ).
  Note: The quality control is specifically manufactured as the positive control.
  Not used for other purposes.
  Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,
  Used in DAB staining on fromalin fixed
  paraffin- embedded Kidney tissue
  Used in Western Blot ,Sample:
  Recombinant HSP90aB1,Human
  BPB 0.01%, NaN3 0.02%. [ STORAGE ]
  Store at 4
  oC for frequent use. Stored at -20oC to -80
  oC in a manual defrost freezer
  for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.