85kDa核孔蛋白(NUP85)多克隆抗体

85kDa核孔蛋白(NUP85)多克隆抗体 

• 来源多抗制备
• 宿主兔
• 效价-
• Ig类型IgG
• 纯化方式抗原特异性亲和纯化
• 85kDa核孔蛋白(NUP85)多克隆抗体标记物无标记物
• **原-
• 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
• 性状液体
• 浓度200ug/ml
• 且适物种-
• 应用WB, ICC, IHC-P, IHC-F, ELISA
• 85kDa核孔蛋白(NUP85)多克隆抗体Polyclonal Antibody to Nucleoporin 85kDa (NUP85)
  Organism Species: Mus musculus (Mouse)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  10th Edition (Revised in Jan, 2014)
  [ PRODUCT INFORMATION ]
  Immunogen: NUP85, Mouse
  Clonality: Polyclonal
  Host: Rabbit
  Immunoglobulin Type: IgG
  Purification: Affinity Chromatography.
  Applications: WB, ICC, IHC-P, IHC-F, ELISA
  Concentration: 200µg/mL
  UOM: 100µg
  85kDa核孔蛋白(NUP85)多克隆抗体[ IMMUNOGEN INFORMATION ]
  Immunogen: Recombinant NUP85 (Gln418~Leu653) with two N-terminal Tags,
  His-tag and T7-tag expressed in E.coli.
  Accession No.: RPB110Mu01
  [ ANTIBODY SPECIFITY ]
  The antibody is a rabbit polyclonal antibody raised against NUP85. It has been
  selected for its ability to recognize NUP85 in immunohistochemical staining and
  western blotting.
  [ APPLICATIONS ]
  Western blotting: 1:50-400
  Immunocytochemistry in formalin fixed cells: 1:50-500
  Immunohistochemistry in formalin fixed frozen section: 1:50-500
  Immunohistochemistry in paraffin section: 1:10-100
  Enzyme-linked Immunosorbent Assay: 1:100-5000
  Optimal working dilutions must be determined by end user.
  [ CONTENTS ]
  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  85kDa核孔蛋白(NUP85)多克隆抗体[ QUALITY CONTROL ]
  Content: The quality control contains recombinant NUP85 (Gln418~Leu653)
  disposed in loading buffer.
  Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
  5uL pe r well when used in enhan ced chemilumes cent (ECL ).
  Note: The quality control is specifically manufactured as the positive control.
  Not used for other purposes.
  Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,
  BPB 0.01%, NaN3 0.02%.
  [ STORAGE ]
  Store at 4
  oC for frequent use. Stored at -20oC in a manual defrost freezer for one
  year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  [ IMAGES ]
  Used in Western Blot, Sample:
  Recombinant NUP85, Mouse
  Used in DAB staining on fromalin fixed
  paraffin- embedded spleen tissue
  PAB110Mu01
  Polyclonal Antibody to Nucleoporin 85kDa (NUP85)
  Organism Species: Mus musculus (Mouse)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  Used in Western Blot, Sample:
  Lane1: Mouse Lung Tissue
  Lane2: Mouse Liver Tissue
  Lane3: Mouse Heart Tissue
  Primary Ab: 2µg/mL Rabbit Anti-Mouse NUP85 Ab
  Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig
  Anti-Rabbit Ab (Catalog: SECab05)