70kDa热休克蛋白1B(HSPA1B)多克隆抗体

70kDa热休克蛋白1B(HSPA1B)多克隆抗体 

• 来源多抗制备
• 宿主兔
• 效价-
• Ig类型IgG
• 纯化方式抗原特异性亲和纯化
• 70kDa热休克蛋白1B(HSPA1B)多克隆抗体标记物无标记物
• **原-
• 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
• 性状液体
• 浓度200ug/ml
• 且适物种-
• 应用WB, ICC, IHC-P, IHC-F, ELISA
• 70kDa热休克蛋白1B(HSPA1B)多克隆抗体Polyclonal Antibody to Heat Shock 70kDa Protein 1B (HSPA1B)
  Organism Species: Bos taurus; Bovine (Cattle)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  9th Edition (Revised in Jul, 2013)
  [ PRODUCT INFORMATION ]
  Immunogen: HSPA1B, Bovine
  Clonality: Polyclonal
  Host: Rabbit
  Immunoglobulin Type: IgG
  Purification: Affinity Chromatography.
  Applications: WB, ICC, IHC-P, IHC-F, ELISA
  Concentration: 200µg/mL
  UOM: 100µg Sample: Recombinant HSPA1B, Bovine
  70kDa热休克蛋白1B(HSPA1B)多克隆抗体[ IMMUNOGEN INFORMATION ]
  Immunogen: Recombinant HSPA1B (Met1~Asp641) expressed in E.coli. USCN Accession No.: RPA301Bo01
  Sequence: The target protein is fused with N-terminal His-Tag and its sequence
  is listed below.
  MGHHHHHHSGSEF-MAKNTAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS
  YVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFRVINDGDK
  PKVQVSYKGE TKAFYPEEISSMVLTKMKEI AEAYLGHPVT NAVITVPAYF NDSQRQATKD
  AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK
  ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA
  SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS
  TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS
  LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT RDNNLLGRFE
  LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ
  EAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW
  LDANTLAEKD EFEHKRKELE QVCNPIISRL YQGAGGPGAG GFGAQGPKGG SGSGPTIEEV
  D
  [ ANTIBODY SPECIFITY ]
  The antibody is a rabbit polyclonal antibody raised against HSPA1B. It has been
  selected for its ability to recognize HSPA1B in immunohistochemical staining and
  western blotting.
  70kDa热休克蛋白1B(HSPA1B)多克隆抗体[ APPLICATIONS ]
  Western blotting: 1:100-400
  Immunocytochemistry in formalin fixed cells: 1:100-500
  Immunohistochemistry in formalin fixed frozen section: 1:100-500
  Immunohistochemistry in paraffin section: 1:50-200
  Enzyme-linked Immunosorbent Assay: 1:100-200
  Optimal working dilutions must be determined by end user.
  [ CONTENTS ]
  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  [ QUALITY CONTROL ]
  Content: The quality control contains recombinant HSPA1B (Met1~Asp641)
  disposed in loading buffer.
  Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
  5uL pe r well when used in enhan ced chemilumes cent (ECL ).
  Note: The quality control is specifically manufactured as the positive control.
  Not used for other purposes.
  Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
  Store at 4
  oC for frequent use. Stored at -20oC to -80
  oC in a manual defrost freezer
  for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.