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- 产品名称：Ⅳ型胶原α2(COL4α2)多克隆抗体
- 产品型号：COL4α2
- 产品展商：HZbscience
- 产品文档：无相关文档
- 发布时间：2016-08-17
- 在线询价
简单介绍
Ⅳ型胶原α2(COL4α2)多克隆抗体是针对COL4a2的兔多克隆抗体。在**组织化学染色和**印迹实验中能识别COL4a2。Ⅳ型胶原α2(COL4α2)多克隆抗体丰富产品组合，其中包括超过 42,000 多种高品质抗体，并配套提供种类齐全的抗体相关产品。 我们的抗体测定结果经全球上千次的引用验证，有助于您在一系列抗体应用中获得优异的实验结果。
产品描述
Ⅳ型胶原α2(COL4α2)多克隆抗体
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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Ⅳ型胶原α2(COL4α2)多克隆抗体标记物无标记物
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**原-
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缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
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性状液体
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浓度200ug/ml
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且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
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Ⅳ型胶原α2(COL4α2)多克隆抗体Polyclonal Antibody to Collagen Type IV Alpha 2 (COL4a2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: COL4a2, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
Ⅳ型胶原α2(COL4α2)多克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant COL4a2 (Ser1487~Ser1703) expressed in E.coli. Accession No.: RPC144Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - SIGY LLVKHSQTDQ
EPM CP VGM NK LW SGY S L LYF EG QE K A H N Q D LG L A GS C LA R FSTMP F LY CN
PGDVCYYASR NDKSYWLSTT APLPMMPVAE DEIKPYISRC SVCEAPAIAI AVHSQDVSIP
HCPAGWRSLW IGYSFLMHTAAGDEGGGQSL VSPGSCLEDF RATPFIECNG GRGTCHYYAN
KYSFWLTTIP EQSFQGSPSA DTLKAGLIRT HIS
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against COL4a2. It has been
selected for its ability to recognize COL4a2 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
Ⅳ型胶原α2(COL4α2)多克隆抗体[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant COL4a2 (Ser1487~Ser1703)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin-embedded Kidney tissue
Used in Western Blot, Sample:
Recombinant COL4a2, Human
