25kDa突触关联蛋白(SNAP25)多克隆抗体

25kDa突触关联蛋白(SNAP25)多克隆抗体 

• 来源多抗制备
• 宿主兔
• 效价-
• Ig类型IgG
• 纯化方式抗原特异性亲和纯化
• 25kDa突触关联蛋白(SNAP25)多克隆抗体标记物无标记物
• **原-
• 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
• 性状液体
• 浓度200ug/ml
• 且适物种-
• 应用WB, ICC, IHC-P, IHC-F, ELISA
• 25kDa突触关联蛋白(SNAP25)多克隆抗体Polyclonal Antibody to Synaptosomal Associated Protein 25kDa (SNAP25)
  Organism Species: Multi-species
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  10th Edition (Revised in Jan, 2014)
  [ PRODUCT INFORMATION ]
  Immunogen: SNAP25
  Clonality: Polyclonal
  Host: Rabbit
  Immunoglobulin Type: IgG
  Purification: Affinity Chromatography.
  Applications: WB, ICC, IHC-P, IHC-F, ELISA
  Concentration: 200µg/mL
  UOM: 100µg
  25kDa突触关联蛋白(SNAP25)多克隆抗体[ IMMUNOGEN INFORMATION ]
  Immunogen: Recombinant SNAP25 (Met1~Gly206) with two N-terminal Tags,
  His-tag and T7-tag expressed in E.coli. Accession No.: RPC955Hu01
  [ ANTIBODY SPECIFITY ]
  The antibody is a rabbit polyclonal antibody raised against SNAP25. It has been
  selected for its ability to recognize SNAP25 in immunohistochemical staining and
  western blotting.
  Note: Full length human SNAP25 is identical in sequence to mouse and rat. 100%
  cross-reactivity of SNAP25 was observed among human, mouse and rat.
  [ APPLICATIONS ]
  Western blotting: 1:50-400
  Immunocytochemistry in formalin fixed cells: 1:50-500
  Immunohistochemistry in formalin fixed frozen section: 1:50-500
  Immunohistochemistry in paraffin section: 1:10-100
  Enzyme-linked Immunosorbent Assay: 1:100-5000
  Optimal working dilutions must be determined by end user.
  [ CONTENTS ]
  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  25kDa突触关联蛋白(SNAP25)多克隆抗体[ QUALITY CONTROL ]
  Content: The quality control contains recombinant SNAP25 (Met1~Gly206)
  disposed in loading buffer.
  Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
  5uL pe r well when used in enhan ced chemilumes cent (ECL ).
  Note: The quality control is specifically manufactured as the positive control.
  Not used for other purposes.
  Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
  Store at 4
  oC for frequent use. Stored at -20oC in a manual defrost freezer for one
  year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  [ IMAGES ]
  Used in Western Blot, Sample:
  Recombinant SNAP25
  Used in DAB staining on fromalin fixed
  paraffin- embedded brain tissue
  PAC955Mi01
  Polyclonal Antibody to Synaptosomal Associated Protein 25kDa (SNAP25)
  Organism Species: Multi-species
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  Used in Western Blot, Sample:
  Lane1: Mouse Spinal Marrow Tissue
  Lane2: Mouse Cerebellum Tissue
  Lane3: Porcine Brain Tissue
  Primary Ab: 1µg/mL Rabbit Anti-Human SNAP25 Ab
  Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig
  Anti-Rabbit Ab (Catalog: SECab05)