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- 产品名称:133kDa核孔蛋白(NUP133)多克隆抗体
- 产品型号:NUP133
- 产品展商:HZbscience
- 产品文档:无相关文档
- 发布时间:2016-08-17
- 在线询价
简单介绍
133kDa核孔蛋白(NUP133)多克隆抗体是针对NUP133的兔多克隆抗体。在**组织化学染色和**印迹实验中能识别NUP133。133kDa核孔蛋白(NUP133)多克隆抗体丰富产品组合,其中包括超过 42,000 多种高品质抗体,并配套提供种类齐全的抗体相关产品。 我们的抗体测定结果经全球上千次的引用验证,有助于您在一系列抗体应用中获得优异的实验结果。
产品描述
133kDa核孔蛋白(NUP133)多克隆抗体
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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133kDa核孔蛋白(NUP133)多克隆抗体标记物无标记物
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**原-
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缓冲液成份磷酸盐缓冲液(pH7.4,含有0.02% NaN3和50%甘油)
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性状液体
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浓度200ug/ml
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且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
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133kDa核孔蛋白(NUP133)多克隆抗体Polyclonal Antibody to Nucleoporin 133kDa (NUP133)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: NUP133, Mouse
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
133kDa核孔蛋白(NUP133)多克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant NUP133 (Leu36~His240) expressed in E.coli. Accession No.: RPB152Mu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - LSLGS AVNSPVLFSP
AGRRSSVSSR GTPTRIFPHH SISESVNYDV RVFGSSLPVK IMEALTMAEA DEQLSVHVDE
GGWACLVCTE KLLIWKIAVS PVTKLSVCKE LQLPPSDFHG SADLVALSYA ATSGEVHSVQ
AVSVMVATKE GSIRYWPSLA REDTYSDTCV DLGGEKMCRF LTAVQGGSFI LSSVGSQLVR
LIPESSGKIH
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against NUP133. It has been
selected for its ability to recognize NUP133 in immunohistochemical staining and
western blotting.
133kDa核孔蛋白(NUP133)多克隆抗体[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant NUP133 (Leu36~His240)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin-embedded Heart tissue
Used in Western Blot, Sample:
Recombinant NUP133, Mouse