血管生成素1(ANGPT1)单克隆抗体
Monoclonal Antibody to Angiopoietin 1 (ANGPT1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: ANGPT1, Human
Clonality: Monoclonal
Clone number: C5
Host: Mouse
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 500µg/mL
UOM: 200µg
血管生成素1(ANGPT1)单克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant ANGPT1 (Arg284~Cys452) with N-terminal His-Tag
expressed in E.coli. Accession No.: RPA008Hu01
[ ANTIBODY SPECIFITY ]
The antibody is a mouse monoclonal antibody raised against ANGPT1. It has been
selected for its ability to recognize ANGPT1 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant ANGPT1 (Arg284~Cys452)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC in a manual defrost freezer for one
year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
血管生成素1(ANGPT1)单克隆抗体[ IMAGES ]
Used in Western Blot, Sample:
Recombinant ANGPT1, Human
