细胞形态: **样
**类型: IgG3
ATCC Number: HB-8248™
数量: 大量
运输方式: 冻存运输
是否是肿瘤细胞: 0
物种来源: 小鼠
生长状态: 悬浮生长
Designations: HT 29/36 [SKI 294/HT 29/36]
HT 29/36细胞Depositors: Sloan-Kettering Institute for Cancer Research
Isotype: IgG3
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human colon adenocarcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. HT 29/36细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Comments: Animals were immunized with a human colon cancer cell line (HT-29, see ATCC HTB-38).
The antibody reacts with human colon adenocarcinoma cells.
It does not react with frozen sections of normal ***** tissues.
This is one of a panel of hybridomas (ATCC HB-8232 and ATCC HB-8240 through ATCC HB-8252) that can be used to diagnose and classify human gastrointestinal tract cancers.
Propagation: HT 29/36细胞ATCC complete growth medium: RPMI 1640 medium with non-essential amino acids and 2% HT, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium, 90%; DMSO, 10%
References: 3882: Cordon-Cardo C, et al. Monoclonal antibodies to human gastrointestinal cancers and hybridoma method of production of the monoclonal antibodies. HT 29/36细胞US Patent 4,579,827 dated Apr 1 1986