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HT 29/36细胞

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  • 产品名称:HT 29/36细胞
  • 产品型号:HT 29/36
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-07-24
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简单介绍
HT 29/36细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。HT 29/36细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述
HT 29/36细胞

细胞形态: **样

**类型: IgG3

ATCC Number: HB-8248™

数量: 大量

运输方式: 冻存运输

是否是肿瘤细胞: 0

物种来源: 小鼠

生长状态: 悬浮生长

Designations: HT 29/36 [SKI 294/HT 29/36]

HT 29/36细胞Depositors: Sloan-Kettering Institute for Cancer Research

Isotype: IgG3

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human colon adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. HT 29/36细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

Comments: Animals were immunized with a human colon cancer cell line (HT-29, see ATCC HTB-38).

The antibody reacts with human colon adenocarcinoma cells.

It does not react with frozen sections of normal ***** tissues.

This is one of a panel of hybridomas (ATCC HB-8232 and ATCC HB-8240 through ATCC HB-8252) that can be used to diagnose and classify human gastrointestinal tract cancers.

Propagation: HT 29/36细胞ATCC complete growth medium: RPMI 1640 medium with non-essential amino acids and 2% HT, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium, 90%; DMSO, 10%

References: 3882: Cordon-Cardo C, et al. Monoclonal antibodies to human gastrointestinal cancers and hybridoma method of production of the monoclonal antibodies. HT 29/36细胞US Patent 4,579,827 dated Apr 1 1986

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