ATCC Number： HB-229™
细胞形态： **样
**类型： IgM
生长状态： 悬浮生长
运输方式： 冻存运输
数量： 大量
是否是肿瘤细胞： 0
物种来源： 大鼠
Designations: MD2
Depositors: RV Rouse
Isotype: IgM
MD2细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus MD2细胞(myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse medullary thymic epithelium
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Comments: Animals were immunized with mouse thymic stromal tissue.
Spleen cells were fused with Sp2/0 myeloma cells.
MD2细胞In the mouse thymus, MD2 is specific for dendritic cells in the medulla.
The antibody cross-reacts with rare cells in the human thymic medulla and faintly with Hassall's bodies.
It also reacts with epithelial elements in a variety of mouse tissues.
Propagation: ATCC complete growth medium: RPMI 1640 medium with non-essential amino acids, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 58334: Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. MD2细胞PubMed: 2461413
