数量： 大量
生长状态： 悬浮生长
运输方式： 冻存运输
ATCC Number： HB-8191™
细胞形态： **样
**类型： IgM
是否是肿瘤细胞： 0
物种来源： 小鼠
Designations: alpha-ACE 3.1.1 [ACE 3.1.1, a-ACE 3.1.1]
Depositors: Wisconsin Alumni Res. Fndn.
Isotype: IgM
alpha-ACE 3.1.1细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against angiotensin converting enzyme (ACE)
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alpha-ACE 3.1.1细胞Tumorigenic: Yes
Comments: Animals were immunized with a crude preparation of rat lung ACE with incomplete Freund's adjuvant.
Spleen cells were fused with P3-NSI/1-Ag4/1 myeloma cells.
The antibody reacts with purified ACE and endothelial cells of mouse, bovine and human origin.
The antibody can be used to isolate endothelial cells from the above species.
Propagation: ATCC complete growth medium: alpha-ACE 3.1.1细胞Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.005 mM 2-mercaptoethanol, 85%; heat-inactivated fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1649: Auerbach R, et al. Monoclonal antibody against angiotensin-converting enzyme: Its use as a marker for murine, bovine, and human endothelial cells. Proc. Natl. Acad. Sci. USA 79: 7891-7895, 1982. PubMed: 6296854
24374: alpha-ACE 3.1.1细胞Auerbach R. Monoclonal antibody to angiotension-converting enzyme and methods of preparing and using same. US Patent 4,582,790 dated Apr 15 1986
