运输方式： 冻存运输
生长状态： 贴壁生长
数量： 大量
器官来源： 肺
ATCC Number： CRL-5900™
相关**： 非小细胞肺癌
细胞类型： 其他细胞类型
年限： stage 4
是否是肿瘤细胞： 0
物种来源： 人
Designations: NCI-H1869 [H1869]
Depositors: AF Gazdar, JD Minna
Biosafety Level: 1
NCI-H1869细胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent, adherent
Organism: Homo sapiens
Morphology:
Source: Organ: lung
Tumor Stage: stage 4
Disease: non-small cell lung cancer
Derived from metastatic site: pleural effusion
Cell Type: squamous cell;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The line is available with the following restrictions: 1. NCI-H1869细胞This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11
D7S820: 8,9
THO1: 6
TPOX: 8,9
vWA: 16
NCI-H1869细胞Age: 58 years
Gender: male
Ethnicity: Caucasian
Comments: The line was established in March 1988.
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
50 pack years.
Propagation: ATCC complete growth medium: ACL-4 medium supplemented with 10% FBS
The base medium for this cell line is ATCC-formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
0.02 mg/ml insulin
0.01 mg/ml transferrin
25 nM sodium selenite
50 nM Hydrocortisone
1 ng/ml Epidermal Growth Factor (do not filter)
0.01 mM ethanolamine
0.01 mM phosphorylethanolamine
100 pM triiodothyronine
0.5% (w/v) bovine serum albumin
10 mM HEPES
0.5 mM sodium pyruvate
2mM L-glutamine(for final conc. of 4.5 mM)
10% FBS
NCI-H1869细胞Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Centrifuge, resuspend cells in fresh medium and dispense into new flasks.
Preservation: RPMI 1640 medium 85%; fetal bovine serum, 10%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. NCI-H1869细胞J. Cell. Biochem. suppl. 24: 1996.
