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LL/2 (LLC1)细胞

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  • 产品名称:LL/2 (LLC1)细胞
  • 产品型号:LL/2 (LLC1)
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-06-28
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简单介绍
LL/2 (LLC1)细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。LL/2 (LLC1)细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述
LL/2 (LLC1)细胞

生长状态: 混合型生长

品系: C57BL

细胞形态: 其他

是否是肿瘤细胞: 0

物种来源: 小鼠

ATCC Number: CRL-1642™

运输方式: 冻存运输

数量: 大量

器官来源: 肺

相关**: 其他**

Designations: LL/2 (LLC1)

LL/2 (LLC1)细胞Depositors: JS Bertram

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Mus musculus

Morphology: rounded - loosely attached or floating


Source: Strain: C57BL

Organ: lung

Disease: Lewis lung carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. LL/2 (LLC1)细胞Please click here for information regarding the specific requirements for shipment to your location.

Applications: Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.

Tested and found negative for ectromelia virus (mousepox).

The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate.

This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.

Tumorigenic: Yes

Antigen Expression: H-2b

Comments: This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.

Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.

The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate.

The cells are reported to be highly tumorigenic, but weakly metastatic in mice.

LL/2 (LLC1)细胞The cells form multilayers in flasks without actually becoming confluent.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Subcultures are prepared by diluting the suspension 1:4 to 1:6. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 21 hrs

Related Products: Recommended mediumLL/2 (LLC1)细胞 (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 1091: Bertram JS, Janik P. Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture. Cancer Lett. 11: 63-73, 1980. PubMed: 7226139

45212: Sharma S, et al. T cell-derived IL-10 promotes lung cancer growth by suppressing both T cell and APC function. J. Immunol. 163: 5020-5028, 1999. PubMed: 10528207

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