细胞类型： T**细胞
数量： 大量
是否是肿瘤细胞： 0
物种来源： 人
运输方式： 冻存运输
生长状态： 悬浮生长
细胞形态： **样
ATCC Number： CRL-8066™
相关**： 其他**
Designations: Mo [Mo T]
Mo [Mo T]细胞Depositors: Genetics Institute, Inc., Regents of the University of California
Biosafety Level: 2 [Cells Contain RETROVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: hairy cell leukemia
Cell Type: T lymphocyte;
Cellular Products: granulocyte macrophage colony stimulating factor (GM-CSF, GM CSF), also known as neutrophil migration inhibitory factor (NIF-T); interferon
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Mo [Mo T]细胞DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,13
D16S539: 11
D5S818: 11,12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 17,18
Gender: male
Comments: A plasmid containing the DNA sequence coding from Mo that codes for CSF is available as ATCC 39754.
Mo cells contain a replication competent genome of Human T Cell Leukemia Virus II (HTLV-II) and two defective HTLV-II genomes.
For long term culture, the cells can be grown in medium with 2% fetal bovine serum.
Propagation: ATCC complete growth medium: Mo [Mo T]细胞The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Atmosphere: 8 to 9% CO2 in air
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
The cells are subcultured every 7 days by centrifugation and division of the cell pellet 1:4 (1:6 maximum).
Do not dilute below 2 X 105 viable cells/ml or allow to exceed 2 X 106 cells/ml.
Culture at 8 to 9% CO2 in air.
The donor suggests using Falcon flasks for optimum growth.
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2005
Mo [Mo T]细胞Recommended serum: ATCC 30-2020
Cell culture tested DMSO: ATCC 4-X
Erythrosin B vital stain solution: ATCC 30-2404
References: 3527: Golde DW, Quan SG. Unique T-lymphocyte line and products derived therefrom. US Patent 4,438,032 dated Mar 20 1984
22772: Clark SC, et al. Method for identification and isolation of DNA encoding a desired protein. US Patent 4,675,285 dated Jun 23 1987
22828: Saxon A, et al. Immunologic characterization of hairy cell leukemias in continuous culture. J. Immunol. 120: 777-782, 1978. PubMed: 344797
23502: Gasson JC, et al. Purified human granulocyte-macrophage colony-stimulating factor: direct action on neutrophils. Science 226: 1339-1342, 1984. PubMed: 6390681
