ATCC Number： HB-197™
数量： 大量
生长状态： 悬浮生长
运输方式： 冻存运输
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 大鼠
**类型： IgG2b
Designations: 2.4G2
Depositors: JC Unkeless
Isotype: IgG2b
Biosafety Level: 1
2.4G2细胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against the Fc gamma receptor (FcRII, CD32)
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Applications: Tested and found negative for ectromelia virus (mousepox).
The antibody can be used to block non-specific binding to Fc gamma bearing cells.
2.4G2细胞The antibody reacts with and immunoprecipitates the 50000 dalton to 70000 dalton Fc gamma receptor on macrophages and Fc gamma bearing lymphoid cells.
Tumorigenic: Yes
Comments: Animals were immunized with the J774 mouse macrophage cell line.
Spleen cells were fused with P3U1 myeloma cells.
The antibody reacts with and immunoprecipitates the 50000 dalton to 70000 dalton Fc gamma receptor on macrophages and Fc gamma bearing lymphoid cells.
The antibody can be used to block non-specific binding to Fc gamma bearing cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; horse serum, 5%; fetal bovine serum, 5%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. 2.4G2细胞Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
References: 1631: Unkeless JC. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J. Exp. Med. 150: 580-596, 1979. PubMed: 90108
1632: Mellman IS, Unkeless JC. 2.4G2细胞Purification of a functional mouse Fc receptor through the use of a monoclonal antibody. J. Exp. Med. 152: 1048-1069, 1980. PubMed: 6158545
1634: Nussenzweig MC, et al. Studies of the cell surface of mouse dendritic cells and other leukocytes. J. Exp. Med. 154: 168-187, 1981. PubMed: 7252426
27575: Yoshikai Y, et al. Clonal expansion of superantigen-reactive T cells is resistant to FK506 in mice with AIDS. J. Virol. 71: 746-749, 1997. PubMed: 8985410
32947: Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913
