运输方式： 冻存运输
生长状态： 悬浮生长
数量： 大量
ATCC Number： HB-275™
细胞形态： **样
**类型： IgG1
是否是肿瘤细胞： 0
物种来源： 小鼠
Designations: CC58
Depositors: CJ Howard
Isotype: IgG1
Biosafety Level: 1
CC58细胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against bovine CD8 (BoCD8)
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Comments: Animals were immunized with bovine lymphocytes and thymocytes.
Spleen cells were fused with NS1 myeloma cells.
CC58细胞The antibody is specific for bovine CD8 T lymphocyte antigen.
It appears to be reactive only with the alpha/beta heterodimer and not the alpha/alpha homodimer.
This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: CC58细胞Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
References: 22775: Hein WR, et al. Epitopes of the T19 lymphocyte surface antigen are extensively conserved in ruminants. Vet. Immunol. Immunopathol. 27: 173-181, 1991. CC58细胞PubMed: 1708546
