细胞类型： B**细胞
生长状态： 悬浮生长
运输方式： 冻存运输
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 小鼠
**类型： IgA; lambda 2 light chain
品系： BALB/c
数量： 大量
ATCC Number： TIB-23™
MOPC 315细胞相关**： 多发性骨髓瘤
Designations: MOPC 315
Depositors: M Cohn
Isotype: IgA; lambda 2 light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphocyte;
MOPC 315细胞Cellular Products: immunoglobulin; monoclonal antibody; against DNP and TNP substituted proteins
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: H-2d
Comments: This line was established from the MOPC 315 plasmacytoma (induced with mineral oil in a BALB/c mouse).
The cells are killed by 10 nM dexamethasone.
Initial recovery from the frozen state is slow, and approximately 7 to 10 days are required before any noticeable cell growth occurs.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: MOPC 315细胞The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1114: Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956
22228: Halpern MS, Coffman RL. Polymer formation and J chain synthesis in mouse plasmacytomas. J. Immunol. 109: 674-680, 1972. PubMed: 5074326
23163: Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. MOPC 315细胞Rev. 52: 631-719, 1972. PubMed: 4555516
