QM7细胞
数量： 大量
生长状态： 贴壁生长
ATCC Number： CRL-1962™
相关**： 纤维肉瘤
年限： 1 to 3 weeks
细胞形态： 成纤维样
运输方式： 冻存运输
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 其他
组织来源： muscle
Designations: QM7 (Quail muscle clone 7)
Depositors: PB Antin
QM7细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Coturnix coturnix japonica
Morphology: fibroblast
Source: Tissue: muscle
Disease: fibrosarcoma
Cell Type: myoblast myoblast; chemically induced
Cellular Products: QM7细胞desmin; cardiac troponin T; cardiac troponin C; skeletal troponin T; skeletal troponin I; alpha tropomyosin; muscle creatine kinase; myosin light chain 2; myosin heavy chain (ventricular form)
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Age: 1 to 3 weeks
Comments: The QM7 cell line was derived from the QT6 fibrosarcoma originally isolated by Moscovici, et al. from a tumor that developed in a bird treated with methylcholanthrene.
QM7 is a serum inducible myogenic cell line.
QM7细胞The cells replicate as myoblasts in medium containing serum.
When switched to medium without serum, the cells cease dividing and fuse to form large multinucleated myotubes.
In the myotube state, the cells express muscle specific proteins such as desmin, cardiac troponin T, cardiac troponin C, skeletal troponin T, skeletal troponin I, alpha tropomyosin.
Mucle creatine kinase, myosin light chain 2 and a ventricular form of myosin heavy chain.
They do not express any known form of alpha actin.
QM7 cells transfect with high efficiency, and are useful for studying many aspects of muscle differentiation and gene expression.
The myoblastic population will become depleted rapidly if the cultures are allowed to become confluent.
To prevent loss of myoblastic cell, cultures should be subcultured before they become confluent and the line should be recloned periodically with selection for myoblastic cells.
Propagation: ATCC complete growth medium: Medium 199 with Earle's BSS, 80%; tryptose phosphate broth, 10%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: The myoblastic population will become depleted rapidly if the cultures are allowed to become confluent. Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin solution. Incubate the flask at room temperature until the cells detach (about 5 minutes).QM7细胞 Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
References: 23002: Antin PB, Ordahl CP. Isolation and characterization of an avian myogenic cell line. Dev. Biol. 143: 111-121, 1991. PubMed: 1985013
70419: Rong S, Sheppard MG. Processes for preparation of Marek's disease virus using continuous avian cell lines. US Patent 6,410,297 dated Jun 25 2002
