7-34-1细胞
ATCC Number: CRL-1945™
运输方式: 冻存运输
生长状态: 悬浮生长
细胞形态: **样
**类型: IgG2a
数量: 大量
是否是肿瘤细胞: 0
物种来源: 小鼠
Designations: 7-34-1
Depositors: CM Warner
Isotype: IgG2a
Biosafety Level: 1
7-34-1细胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against swine leucocyte antigen (SLA) a, c and d haplotypes (cytotoxic)
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Comments: Animals were immunized with peripheral blood mononuclear cells (PMNC) from SLA c haplotype miniature swine.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody react with the MHC class I antigen of swine, and is probably heavy chain specific.
It does not cross-react with PMNC from humans, sheep, goats or mice, but does cross-react with cells from commercial varieties of pigs.
Propagation: ATCC complete growth medium: 7-34-1细胞The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22727: Lie WR, et al. 7-34-1细胞Preparation and characterization of murine monoclonal antibodies to swine lymphocyte antigens. Immunology 64: 599-605, 1988. PubMed: 2459049