R1.G1细胞
细胞类型: T**细胞
是否是肿瘤细胞: 0
物种来源: 小鼠
品系: C58/J
数量: 大量
运输方式: 冻存运输
器官来源: 胸腺
ATCC Number: TIB-44™
相关**: **瘤
细胞形态: **样
生长状态: 悬浮生长
Designations: R1.G1
Depositors: R Hyman
R1.G1细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Organ: thymus
Strain: C58/J
Disease: lymphoma
Cell Type: T lymphocyte;
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Antigen Expression: H-2k; Thy-1.2; TL
Comments: This line was derived from R1.1 (ATCC TIB-42).
The cells are resistant to 0.1 mM 6-thioguanine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22247: R1.G1细胞Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.