MH-S细胞
数量： 大量
ATCC Number： CRL-2019™
生长状态： 混合型生长
器官来源： 肺
年限： 7 weeks
细胞形态： 单核细胞/巨噬细胞
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 小鼠
品系： BALB/c
运输方式： 冻存运输
Designations: MH-S
Depositors: I Mbawuike, HB Herscowitz
Biosafety Level: 2 [Cells may contain SV40 virus ]
Shipped: MH-S细胞frozen
Medium & Serum: See Propagation
Growth Properties: mixed, adherent and suspension
Organism: Mus musculus
Morphology: macrophage
Source: Organ: lung
Strain: BALB/c
Cell Type: macrophage (alveolar);
Cellular Products: interleukin 1 (IL-1)
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Receptors: Fc
Antigen Expression: CD11b (Mac-1); Class II antigens (I-A); T antigen
Age: 7 weeks
Gender: male
Comments: MH-S细胞The MH-S cell line was derived by SV40 transformation of an adherent cell enriched population of mouse alveolar macrophages.
The cells retain many of the properties of alveolar macrophages including typical macrophage morphology.
They are adherent, phagocytic, esterase positive and peroxidase negative.
Lipopolysaccharide (LPS) treatment stimulates IL-1 production.
The cells are capable of suppressing the in vitro plaque forming cell (PFC) response in a cell dose dependent manner.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by transferring floating cells to a centrifuge tube Rinse adherent cells with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. MH-S细胞Add to floating cells collected above and centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22783: Mbawuike IN, Hercowitz HB. MH-S, a murine alveolar macrophage cell line: morphological, cytochemical, and functional characteristics. J. Leukocyte Biol. 46: 119-127, 1989.MH-S细胞 PubMed: 2787372
