NRK-49F细胞
运输方式： 冻存运输
细胞形态： 成纤维样
是否是肿瘤细胞： 0
物种来源： 大鼠
ATCC Number： CRL-1570™
相关**： 正常
器官来源： 肾脏
生长状态： 贴壁生长
数量： 大量
Designations: NRK-49F
Depositors: JE DeLarco
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
NRK-49F细胞Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: fibroblast
Source: Organ: kidney
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Receptors: epidermal growth factor (EGF); multiplication stimulating activity (MSA)
Comments: NRK-49F cells are contact inhibited and very sensitive to transformation by chemicals and viruses.
Cultures should be subcultured before they become confluent.
If allowed to become confluent, the cells will transform.
Propagation: NRK-49F细胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 95%; bovine calf serum, 5%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 22713: de Larco JE, Todaro GJ. Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation. NRK-49F细胞J. Cell. Physiol. 94: 335-342, 1978. PubMed: 304450
23197: Huu D, et al. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J. Bacteriol. 92: 1133-1140, 1966. PubMed: 5926742
32442: Sachsenmeier KF, et al. Transforming growth factor-beta1 inhibits nucleosomal fragmentation in human keratinocytes following loss of adhesion. J. Biol. Chem. 271: 5-8, 1996. PubMed: 8550610
32705: Schultz-Cherry S, Hinshaw VS. Influenza virus neuraminidase activates latent transforming growth factor beta. J. Virol. 70: 8624-8629, 1996. PubMed: 8970987
33046: Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609
33159: Centrella M, et al. NRK-49F细胞Rapid flux in transforming growth factor-beta receptors on bone cells. J. Biol. Chem. 271: 18616-18622, 1996. PubMed: 8702513
