NRbM细胞
运输方式： 冻存运输
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： IgG1
生长状态： 悬浮生长
ATCC Number： CRL-1839™
Designations: NRbM
Depositors: R Wistar, C Cole
Isotype: IgG1
NRbM细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against rabbit IgM
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NRbM细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with rabbit IgM.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with rabbit IgM but not with other rabbit immunoglobulin classes.
Propagation: ATCC complete growth medium: NRbM细胞Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
NRbM细胞References: 58339: Cole C, Wistar R Jr.. Monoclonal antibody anti-rabbit IgM, NR6M. Monoclonal Antibody News 7: 15-16, 1989.
