63D3细胞
ATCC Number： HB-44™
运输方式： 冻存运输
细胞形态： **样
**类型： IgG1
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
数量： 大量
Designations: 63D3
63D3细胞Depositors: JD Capra
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 63D3细胞immunoglobulin; monoclonal antibody; against human monocyte
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Comments: Animals were immunized with purified human peripheral blood monocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a 200000 dalton molecule on human monocytes.
It is not cytotoxic and does not react with human B cells, T cells or endothelial cells.
63D3细胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 936: Ugolini V, et al. 63D3细胞Initial characterization of monoclonal antibodies against human monocytes. Proc. Natl. Acad. Sci. USA 77: 6764-6768, 1980. PubMed: 6935683
