CCD-19Lu细胞 ATCC细胞
细胞类型： 成纤维细胞
细胞形态： 成纤维样
是否是肿瘤细胞： 0
物种来源： 人
数量： 大量
ATCC Number： CCL-210™
相关**： 正常
运输方式： 冻存运输
器官来源： 肺
生长状态： 贴壁生长
年限： 20 years
Designations: CCD-19Lu
Depositors: S Dilworth
CCD-19Lu细胞 ATCC细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: lung
Disease: normal
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. CCD-19Lu细胞
Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11
D5S818: 12
D7S820: 9,11
THO1: 9.3
TPOX: 8
vWA: 17,18
Cytogenetic Analysis: normal female; diploid; stable
Age: 20 years
Gender: female
Ethnicity: Caucasian
Comments: CCD-19Lu细胞The line was derived from normal tissue from patient who died of trauma to the head.
The cells are capable of at least 8 or 9 population doublings beyond the current freeze.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for two minutes at room temperature. Remove trypsin and let the culture stand at 37C for five minutes. Add fresh medium, aspirate and dispense into new flasks. CCD-19Lu细胞Subculture every 6 to 8 days.
