3C7.2细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
ATCC Number： CRL-1959™
生长状态： 悬浮生长
数量： 大量
细胞形态： **样
**类型： IgG1; kappa light chain
Designations: 3C7.2
Depositors: MF Heyworth
Isotype: IgG1; kappa light chain
3C7.2细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Giardia muris trophozoites
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Comments: 3C7.2细胞Animals were immunized with G. muris trophozoites.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
The antibody reacts with G. muris trophozoite surfaces.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;NCTC 109 medium, 10%;(Add 1 mM oxaloacetic acid, 0.45 mM sodium pyruvate, 4 mM L-glutamine, 0.1 mM hypoxanthine, 0.01 mM thymidine and 10 units/ml bovine insulin to this mixture);horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22728: 3C7.2细胞Heyworth MF, et al. Generation and characterization of monoclonal antibodies against Giardia muris trophozoites. Immunology 68: 341-345, 1989. PubMed: 2592009
