LA-4细胞
细胞形态： 上皮样
生长状态： 贴壁生长
品系： A/He
年限： 28 weeks
运输方式： 冻存运输
器官来源： 肺
ATCC Number： CCL-196™
相关**： 其他**
数量： 大量
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 小鼠
LA-4细胞Designations: LA-4
Depositors: GD Stoner
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Organ: lung
Strain: A/He
Disease: adenoma
Cell Type: urethan induced
Permits/Forms: LA-4细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis: Unstable karyotype. Range = 38 to 256. Metacentric chromosomes are noted in about 12% of the cells.
Age: 28 weeks
Comments: Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: LA-4细胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37�C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
References: 23023: Stoner GD, et al. Clonal isolation of epithelial cells from mouse lung adenoma. Cancer Res. 35: 2177-2185, 1975. PubMed: 167947
58031: Stoner GD, et al. LA-4细胞Epithelial cells cultured from mouse lung adenoma: biosynthesis of lecithin and comparison of phospholipids with the lung. Exp. Mol. Pathol. 29: 102-114, 1978. PubMed: 581072
