TE8细胞
生长状态： 悬浮生长
ATCC Number： HB-212™
细胞形态： **样
**类型： IgG2a
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
数量： 大量
Designations: TE8
Depositors: BF Haynes
TE8细胞Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human thymic epithelium (Hassall's bodies)
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Comments: TE8细胞Animals were immunized with human thymic stroma.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with cells forming the outer swirls of Hassall's bodies.
It defines an antigen that is acquired between 14 and 16 weeks of fetal gestation, and marks the terminal stages of epidermal keratinocyte maturation.
The antibody binds specifically to cells forming the outer swirls of Hassall's bodies and also to stratum granulosum of skin epidermis and foreskin.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing: TE8细胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1637: Lobach DF, et al. The human thymic microenvironment. Phenotypic characterization of Hassall's bodies with the use of monoclonal antibodies. J. Immunol. 134: 250-257, 1985. PubMed: 2578044
1638: Haynes BF, et al. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. TE8细胞PubMed: 6200562
