Pfeiffer细胞
年限： ***** *****
生长状态： 悬浮生长
ATCC Number： CRL-2632™
相关**： 其他**
运输方式： 冻存运输
细胞类型： 其他细胞类型
数量： 大量
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 人
**类型： IgG kappa
Designations: Pfeiffer
Depositors: H Ben-Bassat
Pfeiffer细胞Isotype: IgG kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens deposited as human
Morphology: lymphoblast
Source: Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma
Derived from metastatic site: pleural effusion
Cell Type: lymphoblast B lymphocyte;
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Isolation: Isolation date: 1992
Applications: Pfeiffer细胞The cells are negative for Epstein-Barr virus.
The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma (DLCL) with cleaved and non-cleaved nuclei.
Antigen Expression: CD10; Homo sapiens
CD19; Homo sapiens
CD20; Homo sapiens
CD38; Homo sapiens
CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289]
DNA Profile (STR): CSF1PO: 10, 11
D13S317: 11, 12
D16S539: 12
D5S818: 10, 13
D7S820: 8, 12
THO1: 9, 9.3
TPOX: 9
vWA: 17, 18
Amelogenin: XY
Cytogenetic Analysis: The cell line contains several chromosomal abberations including the typical t(14;18)(q32;q21) translocation of follicular lymphomas.
Pfeiffer细胞Age: ***** *****
Gender: male
Ethnicity: White
Comments: The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma (DLCL) with cleaved and non-cleaved nuclei.
The cells are negative for Epstein-Barr virus.
This cell line is a model system for studying non-Hodgkin lymphomas.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Doubling Time: 24 to 30 hrs
Related Products: Pfeiffer细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
